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N. V. Nesterova, V. N. Matveenko, I. A. Samylina, S. V. Kondrashev, N. V. Bobkova, F. Sh. Suleymanova
Identification of phloretin and phlorizine
in the fruits of east apple (Malus Orientalis Uglitzk. ex Jus) and assessment
of the quantitative content of substances of phenolic nature
Abstract
In the complex of
biologically active substances of apple fruits of various species,
dihydrochalcones are of particular interest: phloretin 3-(4-hydroxyphenyl)-1-
(2,4,6-trihydroxyphenyl) propan-1-one (2,4,6,4′-tetrahydroxy dihydrochalcon) and its
glycoside-floridzin (phloretin-2′-glucoside)-(4,6,4-trihydroxy-2-(β-D-glucopyranosyloxy),
with the presence of which it is customary to associate the antidiabetic and
anti-inflammatory effects of apple fruits extracts. The optimal
extraction conditions were selected from fresh and dried fruits of the eastern
apple tree, and 29 phenolic substances were found by HPLC, among which 15
compounds were identified, including phloretin and phlorizin. The
quantification of the content of polyphenolic compounds was carried out by the
Follin–Chicalteu method, the total content of flavonoids was determined by
spectrophotometry under standard conditions. The content of polyphenolic
substances determined in fresh fruits was 12.16%, and in dried fruits – 9.59%.
The total content of flavonoids according to the pharmacopoeial method was
1.28% in fresh fruits and 1.00% in dried fruits. The results can be used to
develop quality standards for fresh and dried raw materials.
Key words: Apple tree East, the fruits of
the tree of the East, polyphenolic substances, flavonoids, dihydrochalcones,
phloretin, phlorizin, HPLC, Folin–Chicalteu method.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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