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N. V. Nesterova, V. N. Matveenko, I. A. Samylina, S. V. Kondrashev, N. V. Bobkova, F. Sh. Suleymanova

Identification of phloretin and phlorizine in the fruits of east apple (Malus Orientalis Uglitzk. ex Jus) and assessment of the quantitative content of substances of phenolic nature


In the complex of biologically active substances of apple fruits of various species, dihydrochalcones are of particular interest: phloretin 3-(4-hydroxyphenyl)-1- (2,4,6-trihydroxyphenyl) propan-1-one (2,4,6,4′-tetrahydroxy dihydrochalcon) and its glycoside-floridzin (phloretin-2′-glucoside)-(4,6,4-trihydroxy-2-(β-D-glucopyranosyloxy), with the presence of which it is customary to associate the antidiabetic and anti-inflammatory effects of apple fruits extracts. The optimal extraction conditions were selected from fresh and dried fruits of the eastern apple tree, and 29 phenolic substances were found by HPLC, among which 15 compounds were identified, including phloretin and phlorizin. The quantification of the content of polyphenolic compounds was carried out by the Follin–Chicalteu method, the total content of flavonoids was determined by spectrophotometry under standard conditions. The content of polyphenolic substances determined in fresh fruits was 12.16%, and in dried fruits – 9.59%. The total content of flavonoids according to the pharmacopoeial method was 1.28% in fresh fruits and 1.00% in dried fruits. The results can be used to develop quality standards for fresh and dried raw materials.
Key words: Apple tree East, the fruits of the tree of the East, polyphenolic substances, flavonoids, dihydrochalcones, phloretin, phlorizin, HPLC, Folin–Chicalteu method.
Moscow University Chemistry Bulletin.
2021, Vol. 62, No. 2, P. 158

Copyright (C) Chemistry Dept., Moscow State University, 2002
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