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Galina
V. Presnova, Tatiana V. Bulko, Victoria V. Shumyantseva, Maya Yu. Rubtsova
Immunosensor
based on screen-printed graphite electrodes modified with gold nanoparticles
and synthetic membrane-like substance for the determination of chloramphenicol
Abstract
Abstract. An electrochemical immunosensor based on screen-printed graphite
electrodes has been developed for the determination of the antibiotic chloramphenicol
in water and milk samples. It has been shown that the immobilization of
chloramphenicol-specific antibodies in a liquid crystal layer of a
membrane-like didodecyldimethylammonium bromide preserves the mobility and
accessibility of active centers of antibodies, and the addition of gold
nanoparticles improves electron transfer from the electrode surface to the
redox centers of horseradish peroxidase used as a label. The limit of detection
of chloramphenicol in water was 0.02 μg/L, in milk – 0.04 μg/L. The
method can be used to determine residual amounts of chloramphenicol in animal
products.
Key words: immunosensors, screen-printed graphite electrodes, chloramphenicol,
gold nanoparticles, horseradish peroxidase, milk
Copyright (C) Chemistry Dept., Moscow State University, 2002
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