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A. A. Kalinina, S. S. Sychev, N. V. Gursky, T. N. Sokolova

Inhibition of intracellular succinate dehydrogenase of bacteria Bacillus Subtilis as a criterion for the participation of an enzyme in the reduction of chloride iodonitrotetrazolium - indicator of cell viability


It is shown in the work that after diffusion of tetrazolium salt to membrane reducing agents of cells of bacteria Bacillus subtilis, the state of the enzyme succinate dehydrogenase affects the rate of recovery. Based on the dependence of the initial rate of reduction of iodonitrotetrazolium chloride by the cellular components of Bacillus subtilis on the concentration of malonic acid, the constant of competitive inhibition of the intracellular enzyme succinate dehydrogenase was quantitatively determined.
Key words: bacteria, iodonitrotetrazolium chloride, kinetics, inhibition, malonic acid, succinate dehydrogenase enzyme.
Moscow University Chemistry Bulletin.
2021, Vol. 62, No. 2, P. 139

Copyright (C) Chemistry Dept., Moscow State University, 2002
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