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Natalia Yu. Chikurova, Anna O. Shemiakina, Daria S. Kryzhanovskaya, Oleg A. Shpigun, Alla V. Chernobrovkina
Comparison of the properties of 3-aminopropyl silica with different
nitrogen content in HILIC mode
Abstract
Abstract. A comparison of 4 amino phases representing 3-aminopropyl silica
batches with different amount of grafted functional groups was made. To
evaluate the chromatographic properties of the adsorbents, the Tanaka test for
hydrophilic stationary phases was used, and the retention of polar substances
of various classes in HILIC mode was also studied. It is shown that even small
changes in nitrogen content between different batches of 3-aminopropyl silica
have a significant effect on the retention of polar analytes. Hydrophilicity of
a substrate was shown to have the greatest effect on its chromatographic
behavior, and its evaluation using the Tanaka test is the basis for a batch
selection either for separation of particular classes of polar substances or
for further modification aiming at obtaining new phases.
Key words: HILIC, 3-aminopropyl silica, substrate, sugars, water-soluble
vitamins, amino acids, organic acids, nitrogenous bases, nucleosides, Tanaka
test
Copyright (C) Chemistry Dept., Moscow State University, 2002
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