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Vitaly G. Grigorenko, Irina P. Andreeva, Elizaveta A. Melnichuk, Pavel A. Levashov

Enzyme-linked immunosorbent assay of autoantibodies against human β1-adrenergic receptor using recombinant antigens


Abstract. E. coli strains have been created as producers of recombinant β1-adreno-receptor epitopes as part of chimeric proteins. The corresponding epitope sequences are located in the C-terminal region of the human heart fatty acid binding protein (hH-FABP) and separated from it by a linker sequence (Gly4Ser)3. A solid-phase enzyme-linked immunosorbent assay (ELISA) for detection of autoantibodies against β1-adrenergic receptor in human blood serum based on a recombinant epitope has been developed. Blood sera of patients (N = 76) with various diagnoses of cardiopathologies and other diseases were analyzed. In some patients with a confirmed diagnosis of cardiovascular diseases, a significantly increased level of autoantibodies to β1-adrenergic receptor was detected, in most cases those with a diagnosis of acute myocardial infarction.
Key words: enzyme immunoassay, autoantibodies, β1-adrenergic receptor, fatty acid binding protein, recombinant antigens, dilated cardiomyopathy
Moscow University Chemistry Bulletin.
2023, Vol. 64, No. 2, P. 203

Copyright (C) Chemistry Dept., Moscow State University, 2002
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