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Olga G. Korotkova, Alexandra M. Rozhkova, Valery Y. Kislitsin, Olga A. Sinitsyna, Yuri A. Denisenko, Maria A. Marochkina, Ivan N. Zorov, Igor A. Shashkov, Aidar D. Satrutdinov, Arkady P. Sinitsyn
New
feed enzyme preparations for the destruction of non-starch polysaccharides and
phytates
Abstract
Annotation. New recombinant strains of Penicillium verruculosum have
been created with a high level of expression of homologous endo-β-1,4-glucanase
II and heterologous phytase A A. niger, as well as heterologous
endo-1,4-β-xylanase E P. canescens and phytase A A. niger,
allowing to obtain highly active feed enzyme preparations (AF) capable of
simultaneously significantly reducing viscosity non-starch polysaccharides, as
well as increase the bioavailability of phosphorus and minerals of grain-based
feed and, thus, increase the digestibility of nutrients of agricultural animal
and poultry feed. Specific AF activities for specific substrates (xylanau,
β-glucan, phytate) were studied, the qualitative and quantitative
component composition of new AF was determined. On the basis of a new enzyme
preparation containing homologous endo-β-1,4-glucanase II and heterologous
phytase A A. niger, a recipient strain ∆nia D
– was obtained, into which heterologous endo-1,4-β-xylanase E P. canescens
was successfully cloned and, thus, a promising producer of three target enzymes
was obtained simultaneously.
Key words: non-starch polysaccharides (NPS), phytase,
endo-β-1,4-glucanase, endo-1,4-β-xylanase, enzyme preparation
Copyright (C) Chemistry Dept., Moscow State University, 2002
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