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Olga G. Korotkova, Alexandra M. Rozhkova, Valery Y. Kislitsin, Olga A. Sinitsyna, Yuri A. Denisenko, Maria A. Marochkina, Ivan N. Zorov, Igor A. Shashkov, Aidar D. Satrutdinov, Arkady P. Sinitsyn

New feed enzyme preparations for the destruction of non-starch polysaccharides and phytates


Annotation. New recombinant strains of Penicillium verruculosum have been created with a high level of expression of homologous endo-β-1,4-glucanase II and heterologous phytase A A. niger, as well as heterologous endo-1,4-β-xylanase E P. canescens and phytase A A. niger, allowing to obtain highly active feed enzyme preparations (AF) capable of simultaneously significantly reducing viscosity non-starch polysaccharides, as well as increase the bioavailability of phosphorus and minerals of grain-based feed and, thus, increase the digestibility of nutrients of agricultural animal and poultry feed. Specific AF activities for specific substrates (xylanau, β-glucan, phytate) were studied, the qualitative and quantitative component composition of new AF was determined. On the basis of a new enzyme preparation containing homologous endo-β-1,4-glucanase II and heterologous phytase A A. niger, a recipient strain ∆nia D was obtained, into which heterologous endo-1,4-β-xylanase E P. canescens was successfully cloned and, thus, a promising producer of three target enzymes was obtained simultaneously.
Key words: non-starch polysaccharides (NPS), phytase, endo-β-1,4-glucanase, endo-1,4-β-xylanase, enzyme preparation
Moscow University Chemistry Bulletin.
2023, Vol. 64, No. 2, P. 178

Copyright (C) Chemistry Dept., Moscow State University, 2002
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