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M. A. Tagirova, N. L. Eremeev, A. N. Vaneev, N. G. Balabushevich, A. D. Aleksashkin, E. A. Zaitseva, N. L. Klyachko
Obtaining a
two-layer polyionic bienzyme complex of superoxide dismutase 1 and catalase of
minimal size
Abstract
The factors
affecting the sizes of two-layer polyionic bienzyme SOD1 / catalase complex/
conjugates (the nature and concentration of buffers, pH, enzymes initial molar
ratios) were studied. The optimal conditions for obtaining conjugates of the
minimal size are determined. The conjugates obtained under these conditions
demonstrate a relatively high storage stability of both enzymes
Key words:
Reactive oxygen species, nanozymes, superoxide dismutase (SOD1), catalase,
two-layer polyionic bienzyme complex.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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