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M. M. Veselov, N. I. Kolomoec, A. R. Blinova, M. V. Efremova, Yu. V. Chudosay, A. N. Prusov, A. O. Zhigachev, Y. I. Golovin, N. L. Klyachko
Biocatalysis of α-chymotrypsin – Bowman–Birk inhibitor pair immobilized onto hybrid
magnetite-gold nanoparticles management under exposure of low-frequency
magnetic field
Abstract
Dumbbell-like
hybrid magnetite-gold magnetic nanoparticles was used to immobilize two
proteins: α-chymotrypsin and Bowman–Birk
inhibitor. It was shown that under exposure of low-frequency 50 Hz magnetic
field the activity of chymotrypsin bounded to inhibitor dramatically decrease
depending on magnetic field strength. This effect is supposed to be the consequence
of aggregation of magnetic nanoparticles under magnetic field action.
Key words:
magnetic nanoparticles, enzyme immobilization, low-frequency magnetic field,
nanoparticles aggregation, biocatalysis regulation.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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