|
I. M. Le-Deygen, P. V. Mamaeva, A. A. Skuredina, E. V. Kudryashova
New details on
the interaction of mannose-modified chitosan with lectin concanavalin a
according to spectral methods
Abstract
The possibility
of comprehensive study of the interaction of receptors with ligands on the
example of concanavalin A lectin and 5 kDa chitosan modified with D-mannose by
spectral methods of analysis was demonstrated. A convenient method for the
synthesis and purification of chitosan mannose is proposed. An analytically
significant decrease in the intensity of the absorption band of Amide II was
found in the IR spectra of the receptor normalized to Amide I upon binding of
chitosan-mannose. The values of the dissociation constants of the complexes are
5,5(0,3)∙10-5M, the results confirm the data of polarization fluorescence
analysis.
Key words:
lectins, ligand-receptor interaction, chitosan, FTIR.
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
