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A. N. Stavrianidi*, E. A. Stekolshchikova, P. N. Turova, I. A. Rodin, O. A. Shpigun
 (Division of Analytical Chemistry; *e-mail: stavrianidi. andrey@gmail. com)

Quantitative analysis of multicomponent system for liquid chromatography mass spectrometry determination of diosgenin, dioscin and protodioscin in plant extracts of Tribulus terrestris


An approach of simultaneous detection of dioscin, diosgenin and protodioscin in extracts from plant material was developed on the basis of liquid chromatography/mass spectrometry (limits of detection were in the range from 5 to 20 ng/ml). Analysis of extracts was carried out using a reversed-phase chromatography with C18 sorbent followed by electrospray ionization in positive ion mode and tandem mass-spectrometric detection in positive multiple ion monitoring mode. For quantification peak areas in extracted ion chromatograms were used in external calibration and quantitative analysis of multicomponent system approaches. It was shown that difference in the results was lower than 10 %. The proposed approach was tested during the analysis of ethanolic infusion and water-methanolic extract from the aerial parts of plant Tribulus terrestris.
Key words: high performance liquid chromatography/mass spectrometry, electrospray ionization, extraction, vegetable raw materials, Tribulus terrestris, saponins.
Moscow University Chemistry Bulletin.
2017, Vol. 58, No. 3, P. 144

Copyright (C) Chemistry Dept., Moscow State University, 2002
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