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A. N. Stavrianidi*, E. A. Stekolshchikova, P. N. Turova, I. A. Rodin, O. A. Shpigun
(Division of Analytical Chemistry; *e-mail: stavrianidi. andrey@gmail. com)
Quantitative analysis
of multicomponent system for liquid chromatography mass spectrometry
determination of diosgenin, dioscin and protodioscin in plant extracts of Tribulus
terrestris
Abstract
An approach of
simultaneous detection of dioscin, diosgenin and protodioscin in extracts from
plant material was developed on the basis of liquid chromatography/mass
spectrometry (limits of detection were in the range from 5 to 20 ng/ml).
Analysis of extracts was carried out using a reversed-phase chromatography with
C18 sorbent followed by electrospray ionization in positive ion mode and tandem
mass-spectrometric detection in positive multiple ion monitoring mode. For
quantification peak areas in extracted ion chromatograms were used in external
calibration and quantitative analysis of multicomponent system approaches. It
was shown that difference in the results was lower than 10 %. The proposed
approach was tested during the analysis of ethanolic infusion and
water-methanolic extract from the aerial parts of plant Tribulus terrestris.
Key words: high
performance liquid chromatography/mass spectrometry, electrospray ionization,
extraction, vegetable raw materials, Tribulus
terrestris, saponins.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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