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A. N. Malyavko, O. A. Petrova, M. I. Zvereva, O. A. Dontsova
Development
of a system to test for the presence of any nontelomeric nucleotide on the
3’-chromosomal end
Abstract
Amount of telomeric repeats in the telomeres correlated with
proliferative potential eukaryotic cells. The lengthening of telomeres and
telomerase work is regulated by numerous ways. One of this way is based on the
elongation of the 3′-end of chromosome for additional nucleotide that is
not part of the sequence of the telomeric repeat. This work presents an
approach for testing the presence of an additional nontelomeric nucleotide at
the 3′-end of the G-strain of chromosomes. The applicability of this
method on the example of thermotolerant yeast H. polymorpha was shown.
The possibility of modifications of the method to test additional nucleotides
at the 3’-end of the chromosomes of other organisms is discussed.
Key words: telomerase,
telomeres, H. polymorpha, test of terminal nucleotide on 3′-end of a
chromosome, the regulation of telomere length.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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