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Y. A. Polyakova, I. A. Ananieva, E. N. Shapovalova, A. G. Majouga, O. A. Shpigun
Separation
of water soluble vitamins on silica modified L-cysteine-stabilized gold
nanoparticles by HPLC
Abstract
Prepared silica functionalized with 3-aminopropyltriethoxysilane and
L-cysteine-stabilized gold nanoparticles (SiO2–NH2–Au–L-cysteine).
The influence of the content of acetonitrile in the mobile phase and pH on the
retention and separation selectivity 8 vitamins was studied. Chromatographic
conditions allowing to separate mixtures of vitamins C, B3, B12, B5 and B1, B2,
B6, B10 were suggested. The possibility of separating a mixture of vitamins C,
B3, B12, B5 for 8 min, and mixtures of vitamins B1, B2, B6, B10 in 12 min. on
the synthesized stationary phases SiO2–NH2–Au–L-cysteine
in isocratic elution was shown.
Key words: HPLC,
modified silica, gold nanoparticles, L-cysteine, water soluble vitamins.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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