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V. I. Beketov, R. D. Voronina, N. B. Zorov
Fluorimetric determination of amino acids and photochemical
stability of the products of their reaction with orto-phthalic
aldehyde under irradiation of a high-power pulsed laser.
Abstract
The
possibility of high-sensitive determination of amino acids was investigated
with the use of fluorescence of their derivatives obtained by reaction of
aqueous solution of ortho-phthalic dialdehyde and nucleophilic additives:
potassium cyanide, sodium sulfite and N-acetyl-L-cysteine. With glycine as an
example, it was shown that chemical stability of yielded highly fluorescent
compounds (substituted isoindoles) and their photochemical stability under
irradiation of a powerful pulsed laser is substantially higher than the ones of
widely used reagent for determination of amino acids - the basic aqueous
solution of ortho-phthalic dialdehyde and 2-mercaptoethanol. The analytical
characteristics of the newly developed reagents are the same or better than of
the standard one.
Key
words: amino
acids, fluorimetric determination, substituted isoindoles, pulsed laser
excitation, photochemical stability.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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