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N. N. Ugarova, G. Yu. Lomakina, T. A. Perevyshina, E. V. Otrashevskaya, S. V. Chernikov
In process control of BCG vaccine cell
viability by bioluminescent ATP assay
Abstract
The BCG vaccine based on a live strain of Mycobacterium
bovis BCG is widely used for the immunoprophylaxis of tuberculosis. One of
the BCG vaccine key parameters is its cell viability
(specific activity – the number of colony forming units, CFU ), which is
traditionally defined by microbiological method. In this work, the rapid and
selective bioluminescent method of intracellular ATP assay was used to control
the BCG vaccine viability at various stages of the vaccine production. It
permitted to reduce the analysis time from 28 days up to 1 hour. The
correlation was shown between the viability of liquid BCG vaccine, measured by
microbiological method and calculated using the content of intracellular ATP,
as well as the correlation between the CFU value for the lyophilized BCG
vaccine and the ATP content in liquid vaccine before lyophilization.
Key words: BCG vaccine, ATP assay, cell viability, tuberculosis, colony forming units (CFU)
Copyright (C) Chemistry Dept., Moscow State University, 2002
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