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O. S. Savinova, I. N. Zorov, D. V. Vasina, A. P. Sinitsyn, T. V. Fedorova

The minor recombinant laccase isozymes of Trametes hirsuta 072: obtaining and properties


Laccases (EC are multicopper polyphenol oxidases found in various organisms, in particular, in fungi. Fungal laccases are encoded by multigenic families, which can contain up to 17 genes. However, not all isozymes can be obtained from native producers. Previous studies have shown that the filamentous fungus Penicillium canescens is a promising object for the heterologous expression of various laccase isozymes of Trametes hirsuta 072. In this work, the cultivation conditions of P. canescens strains, recombinant producers of T. hirsuta heterologous minor laccase, have been optimized. Optimization of rLacD and rLacF purification method increased their yield and specific activity of rLacD. In addition, the melting points of minor laccase isoenzymes were measured and the glycosylation of isoenzymes was studied.
Key words: laccase, isozymes,Trametes hirsuta, Penicillium canescens, properties.
Moscow University Chemistry Bulletin.
2019, Vol. 60, No. 4, P. 232

Copyright (C) Chemistry Dept., Moscow State University, 2002
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