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P. O. Volkova, A. V. Alekseev, A. A. Dzhatdoeva, E. V. Proskurnina, Yu. A. Vladimirov
Quantitation
of lipid hydroperoxides by enhanced chemiluminescence
Abstract
For the determination of lipid hydroperoxides, an analytical procedure
was proposed based on enhanced chemiluminescence. The analytical system
consists of a lipid, Fe(II), and coumarin C-525 as an enhancer of
chemiluminescence. The lipid hydroperoxides were determined with spiked
solutions using tert-butyl hydroperoxide as an internal standard. The
analytical procedure provides a detection limit as low as 164 nM. The accuracy
verification was performed with iodometric titration. The assay was used for
the determination of total lipid hydroperoxides in food.
Key words: lipid
hydroperoxides, lipid peroxidation, enhanced chemiluminescence, coumarin C-525.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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