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N. Yu. Ignatieva, N. A. Danilov, V. V. Lunin, M. V. Obrezkova, S. V. Averkiev, T. I. Chaikovskii
The
alteration of thermodynamic characteristics of corneal tissue collagen
denaturation as the result of the non-enzymatic glycation
Abstract
The study was intended to
investigate the thermal stability of scleral and corneal tissues subjected to
treatment by ribose, threose and glyceraldehyde in vitro. The thermal
transition temperature and enthalpy of cross-linking collagen fibres were
obtained by differential scanning calorimetry (DSC). The resistance of tissues
by trypsine treatment was defined too after heating in the furnace of the
DSC-device. It was shown that the denaturation temperature of scleral and
corneal samples subjected to treatment by cross-linking agents increased, but
the enthalpy decreased. It is suggested that cross-linking formation in
collagen matrix of cornea and sclera prevents complete collagen denaturation if
the temperature doesn’t go up to 110°C.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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