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S. N. Bendrysheva, N. Yu. Ragozina, D. A. Nedosekin, M. A. Proskurnin, U. Pyell

Optimization of instrumental parameters of the thermal lens detector for capillary electrophoresis

Abstract

The main instrumental parameters of the crossed-beam thermal lens detector for capillary electrophoresis are considered. Optimum values of the photodetector aperture size, distance between the capillary and the probe and excitation beam waists, chopper frequency are calculated from the thermal lens theory and confirmed experimentally. Under optimized conditions, the separation of 2-, 3-, 4-nitrophenols is carried out. The limits of detection (S/N=3) for nitrophenols are n106 n107 mol L1. For these test substances the limits of detection were improved by a factor of 30 compared to conventional UV-photometric detection under the same conditions.
Moscow University Chemistry Bulletin.
2005, Vol. 46, No. 6, P. 411
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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