Natalya Nikolaevna Ugarova
CHEMICAL ENZYMOLOGY DIVISION
LABORATORY OF PHYSICO-CHEMICAL ASPECTS OF
ENERGY BIOCONVERSION
Bioluminescence: Principles and Applications
Date of Birth: November 22, 1936
Education: 1959 - Faculty of Chemistry, Moscow State University
Master's Degree in Physical Chemistry
1966 - Ph.D.in Chemical Kinetics, Moscow State University
1982 - Doctor of Science in Chemical Kinetics,
Moscow State University
1983 - Professor in Chemistry
Research and Professional Experience:
1959-1970: Junior research worker, Faculty of Chemistry,
Moscow State University
1970-1980: Senior research worker, Faculty of Chemistry,
Moscow State University
1988-present: Head of Laboratory, Moscow State University
Membership Biochemical Society of Russia
Scientific Councils of Moscow State University,
International Advisory Committee on Bioluminescence
and Chemiluminescence
Organizing Committees of International
Conferences on Biocatalysis and Enzyme Engineering
Awards: ISF (Soros) Grants, 1993-1994
Grants of Russian Science Foundation, 1989-1994
Wellcome Trust Collaboration Grant, 1993-1995
Award of Russian Scientific Foundation, 1994
Publications: more than 200 publications in international and
russian Journals including 20 books and reviews,
and more than 20 russian patents
Field of Interest: Enzyme mechanism and enzyme kinetics,
Bioluminescence and bioluminescent analysis
in medicine, ecology and biotechnology
Topics for scientific lectures:
Bioluminescence and bioluminescent analysis
Kinetics and mechanism in firefly bioluminescent
enzyme system
RECENT PUBLICATIONS:
- L.Yu. Brovko, O.A. Gandelman and N.N. Ugarova. Firefly bioluminescence.
Fluorescence studies of luciferase interactions with oxyluciferin and its
analogs. In: Biological luminescence, eds. B. Jezowska-Trzebiatowska,
B. Kochel, J. Slawinski and W. Strek. World Scientific. pp. 292-308 (1990).
- N.N. Ugarova. Research on bio- and chemiluminescence in the Soviet Union.
J.Biolum.Chemilum. 6, 137-138 (1991).
- A.I. Titov, N.A. Romanova, T.M. Danilova, L.Yu. Brovko, N.N. Ugarova and
P.I. Tolstykh. Bioluminescent method for assessment of microflora
susceptibility to antibiotics. Lab.Delo (USSR) N 10, 61-66 (1990)
(in Russian).
- G.D. Kutuzova, E.A. Skripkin, N.G. Belogurova, G.A. Skryabin, N.N. Ugarova
and S.D. Varfolomeev. New type of synthesis induction of recombinant proteins.
Low-temperature induction of expession of firefly luciferase genein E.coli.
Dokl.AN.SSSR 314, 757-761 (1990) (in Russian).
- L.Yu. Brovko, I.Yu. Trdatyan and N.N. Ugarova. Optimization of
bioluminescent methods of biomass assay. Prikl.biochem.microbiol. (USSR)
27, 134-141 (1991) (in Russian).
- N.N. Ugarova, Ya.U. Vozny, G.D. Kutuzova and E.I. Dementieva.
Bioluminescent assay of b-galactosidase using D-luciferin-O-galactosidase.
In: Bioluminescece and Chemiluminescence. Current Status, Proc. VI
Int.Symp.Biolum.Chemilum., ed. Ph. Stanley, and L. Kricka, Yohn Wiley & Sons,
pp.511-514 (1991).
- L.Yu. Brovko, N.A. Romanova and N.N. Ugarova. Bioluminescent assay of
bacterial intracellular AMP, ADP, and ATP with the use of a coimmobilized
three-enzyme reagent (adenylatekinase, pyruvate kinase, and firefly
luciferase). Anal.Biochem. 220, 410-414 (1994).
- O.A. Gandelman, L.Yu. Brovko, A.Yu. Chikishev, A.P. Shkurinov and
N.N. Ugarova. Investigation of the interaction between firefly luciferase
and oxyluciferin or its analogues by steady state and subnanosecond
time-resolved fluorescence. J.Photochem.Photobiol. B:Biol., 22, 203-209
(1994).
- L.Yu. Brovko, O.A. Gandelman, T.E. Polenova and N.N. Ugarova. Kinetics
of bioluminescence in the firefly luciferin-luciferase system.
Biochemistry (Moscow) 59, 195-201 (1994).
- G.D. Kutuzova, O.V. Leont'eva, E.A. Skripkin, and N.N. Ugarova. Kinetics
of expression of luciferase genes from the fireflies Photinus pyralis and
Luciola mingrelica in Escherichia coli. 1. Kinetics of conversion of
recombinant luciferase into enzymatically active conformers. Biochemistry
(Moscow) 59, 77-84 (1994).
- O.V. Leont'eva, G.D. Kutuzova, V.B. Turovetskii and N.N. Ugarova.
Kinetics of expression of liciferase genes from the fireflies Photinus
pyralis and Luciola mingrelica in Escherichia coli. 2. Role of pH in the
biosynthesis of proteins and their transformation into active conformers.
Biochemistry (Moscow) 59, 85-88 (1994).
- N.N. Ugarova. Bioanalytical applications of firefly luciferase (review).
Appl.Biochem.Microbiol. (Moscow) 29, 135-144 (1993).
- N.N. Ugarova, L.Yu. Brovko and G.D. Kutuzova. Bioluminescence and
bioluminescent analysis: recent development in the field. Biochemistry
(Moscow) 58, 976-992 (1993).
- A.F. Dukhovich, A.I. Efimov, A.A. Shchegolev, A.V. Levashov and
N.N. Ugarova. Firefly luciferase inactivation by long chain choline derivatives.
Biochemistry (Moscow), 58, 648-654 (1993) (in Russian).
- J.H. Devine, G.D. Kutuzova, V.A. Green, N.N. Ugarova and T.O. Baldwin.
Luciferase from east european firefly Luciola mingrelica: cloning and
nucleotide sequence of the cDNA, overexpression in Esherichia coli and
purification of the enzyme. Biochim.Biophys.Acta, 1173, 121-132 (1993).
- N.A. Romanova, L.Yu. Brovko, M.A. Sepulveda-Bessera and N.N. Ugarova.
Change in adenine nucleotide pool in E.coli 1257 cells under low-intensity
He-Ne laser illumination. Biochemistry (Moscow), 58, 230-236 (1993).
- O.A. Gandelman, L.Yu. Brovko, N.N. Ugarova, A.Yu. Chikishev and
A.P. Shkurimov. Oxyluciferin fluorescence is a model of native bioluminescence
in the firefly luciferin-luciferase system.
J.Photochem.Photobiol. B:Biol. 19, 187-191 (1993).
- G.D. Kutuzova, E.I. Dementieva, T.O. Baldwin and N.N. Ugarova. Recombinant
luciferase of firefly Luciola mingrelica. Cloning, superexpression and
production of homogeneous enzymepreparation. Biotechnologiya (Moscow)
N 5, 43-51 (1992).
- A.F. Dukhovich, V.M. Morozov and N.N. Ugarova. Effect of substrates on
the stability of firefly luciferase incorporated in phosphatidylcholine
liposomes. Biochemistry (Moscow), 57, 1179-1182 (1992).
- L.Yu. Brovko, E.I. Dementieva, E.A. Aronova, P.V. Yevseev and N.N. Ugarova.
Bioluminescent analysis of b-galactosidase and its application for
immunoassay and DNA-probes. Anal.letters 27, 2949-2959 (1994).
- A.F. Dukhovich, V.M. Morozov and N.N. Ugarova. Modulation by substrate,
adenosine 5'-triphosphate, lipid-protein interactions in firefly
luciferin-luciferase system. Biol.membr. (Moscow) 11, 614-619 (1994)
(in Russian).
Chemical Enzymology Division, Chemistry Department,
Moscow State University, Moscow 119899, Russia
Phone: (095)939-2660
Fax: (095)939-3589/2742
E-mail:
UNN@enzyme.chem.msu.su
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